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The Mediterranean region under climate change

 | 
Jean-Paul Moatti
, 
Stéphane Thiébault

Part 3. Adaptation, resilience, conservation of resources and prevention of risk

Sub-chapter 3.5.4. Rethinking the management of mycorrhizal soil infectivity to restore Mediterranean and tropical forest ecosystems

Robin Duponnois, Lahcen Ouahmane, Mohamed Hafidi et Yves Prin

Texte intégral

Introduction

1Desertification, one of the main causes of climate change, generally results from a variety of factors, including climatic variations and human activities. Among the man-mediated degradative activities, deforestation is considered to have a major impact by causing extinction, changes to climatic condition, desertification and the displacement of local populations (Defries et al. 2007).

2Forest cover is an important source of protection from soil degradation and deforestation which impacts population structure, successional patterns and species diversity, generally inducing degradations in the physico-chemical and biological soil properties (Requena et al. 2001). These changes can be recorded in microbial functional capacity (microbial metabolism, biomass and composition, enzymatic activities and soil organic matter flux) which is mainly involved in soil quality and function (Chaer et al. 2009). Many studies have also shown that deforestation and soil cultivation alter soil microbial community structure (Bossio et al. 2005) and may lead to reduction in microbial biodiversity (Chaer et al. 2009).

3Among components of soil microbiota, mycorrhizal fungi are known to be essential key components of sustainable soil-plant systems, especially in arid ecosystems (Duponnois et al. 2011). The mycorrhizal symbiosis mobilizes and transports nutrients to roots (Smith & Read, 2008), reduces water stress (Augé, 2001) and improves soil aggregation in eroded soils (Caravaca et al. 2002). It has also been reported that arbuscular mycorrhizal (AM) fungi affect the diversity of plant communities (van der Heijden et al. 1998)) and influence relationships between plants (van der Heijden et al. 1998).

4Since trees are a primary source of protection from soil degradation, many afforestation programs have been undertaken but with generally low performance in terms of productivity and seedling survival after outplanting (Duponnois et al. 2005a). These deficiencies have usually been recorded in Mediterranean semi-arid areas known to have low bioavailable phosphate content and high phosphate retention capacities (Duponnois et al. 2011). This environmental context represents the most favourable conditions for AMF’s potential to increase plant growth without any mineral fertilizers (Rodriguez & Sanders, 2015). Unfortunately, this microbial resource has been largely neglected despite numerous studies focused on this symbiosis.

5The native inoculum potential of AM fungi in arid and semi-arid Mediterranean ecosystems is generally limited which, in turn, prevents plant establishment and growth (Smith& Read 2008). It is necessary to apply mycorrhizal inoculation technologies or to manage native AM fungus communities to replace or reinforce the mycorrhizal potential in these degraded areas (Duponnois et al. 2011).

Approaches to AM application in forestry practices

6This chapter aims to describe different practical approaches to integrate the AM symbiosis in forestry practices through a “reductionist” approach (also named Controlled mycorrhization) (Inoculation of optimized AM fungal strains to improve the plant growth in unfriendly conditions) or a “holistic” approach (Suitable management of AM fungal diversity for ensuring AM fungi–dependent ecosystem services) (Rodriguez & Sanders, 2015). Each of these cultural practices will be illustrated by results from field experiments performed in Mediterranean and tropical areas.

The “reductionist” approach (controlled mycorrhization)

7In recent decades, considerable research has been made by using specific mycorrhizal fungal strains to enhance outplanting performances with forest tree species (Caravaca et al. 2002). Hence, numerous studies have reported the beneficial effects on plant growth resulting from AM fungal inoculation during the nursery plantation. Among all the AM fungal strains tested in these experiments, Rhizophagus irregularis has attracted great interest because of (1) its world-wide distribution, (2) its high genetic variability and variation in effects on plant growth and (3) its ability to be produced in an in vitro system.

8Some of its impacts on the growth of different tree species in controlled conditions are reported in table 1. Most of these experiments have been performed in controlled conditions and few studies have clearly demonstrated the benefits of fungal inoculation in the field. The degree of mycorrhizal responses on a reafforestation site depends on the status of fungal colonization at planting, and the persistence of introduced fungi and other biotic and abiotic factors at the planting site (Duponnois et al. 2011).

Table 1. Impact of R. irregularis on the growth of tree species in controlled conditions after different times of cultivation

Table 1. Impact of R. irregularis on the growth of tree species in controlled conditions after different times of cultivation

(1) (Shoot biomass of mycorrhizal plants / Shoot biomass of non mycorrhizal plants) x 100. (2) (Root biomass of mycorrhizal plants / Root biomass of non mycorrhizal plants) x 100.

9Hence, the use of AM fungi and plants adapted to the local environmental conditions may be a prerequisite for the success of reafforestation programmes (Duponnois et al. 2005a). The potential effect of mycorrhizal inoculation with native AM fungi on the survival rates and early growth performance in the field of Mediterranean tree species (i.e. cypress, carob) has been assessed in a few studies (Manaut et al. 2015). The results showed the high potential of this approach by sustainably improving the growth and nutrient status of both tree species and also by inducing a positive soil microbial environment for nutrient cycling and environmental stress resistance (figs. 1 & 2).

The “holistic” approach

10It has been reported that certain shrubs react positively to the survival and growth of other neighboring plant species by creating a better environmental habitat with low stresses from high radiation and temperature as well as from soil nutrient and moisture deficiencies (Callaway & Walker, 1997) named “the nurse-plant syndrome” (Niering et al. 1963). The ecological facilitation between plant species results in the patchy distribution of the vegetation commonly observed in Mediterranean areas, especially in degraded ecosystems (Callaway & Walker, 1997).

11Hence it has been suggested that the use of nurse plants as planting microhabitats in Mediterranean degraded ecosystems could promote the survival and development of native tree species and constitute an alternative reforestation technique compared to the standard practices (Duponnois et al. 2011). The “fertility islands” or “resource islands” (Schlesinger et al. 1996) resulting from the establishment of these nurse plants show a higher arbuscular mycorrhizal (AM) soil infectivity compared to the adjacent soil away from plant influence (Duponnois et al. 2011), which can improve plant growth and survival in arid conditions, by increasing the supply of nutrients to the plants (especially for soil P uptake) (Smith & Read, 2008), enhancing soil aggregation in eroded soils (Caravaca et al. 2002) and reducing water stress (Augé, 2001).

12After three years’ plantation, it was reported that the association between C. atlantica and a nurse plant, L. stoechas, enhanced the growth of C. atlantica and provided better soil microbial characteristics compared to the control treatment (fig. 3) (Duponnois et al. 2011). AM mycelium network, total microbial activity, dehydrogenase activity, phosphate-solubilizing fluorescent pseudomonads and N, P nutrient uptake by C. atlantica, were significantly higher in the presence of L. stoechas. This pioneer shrub facilitated the early establishment of Cypress seedlings by improving soil microbial characteristics and AM fungus community development. Since the facilitative effect of one plant species on another increases with abiotic stress (Callaway, 1995), the benefits of this technique would be useful in reforestation programs undertaken to rehabilitate degraded areas in the Mediterranean region (Duponnois et al. 2011). Other shrub species have been identified for their potential nursing effects on Mediterranean tree species (fig. 4).

Figure 1
Height and collar diameter of carob outplants in the field, either inoculated with AM fungi (
) or non-inoculated (control ). An asterisk indicates a significant (P < 0.05) difference between the two treatments for a given year (From Manaut et al. 2015).

Figure 2
Cumulative mortality of carob outplants in the field, either inoculated with AM fungi (
) or non-inoculated (control ) during the three years of plantation (From Manaut et al. 2015).

Figure 3
Time course changes in plant height (expressed in cm) of
C. atlantica outplants growing under natural conditions in the High Atlas Mountains (Morocco), either non-inoculated (Control) () or associated with L. stoechas plants (). Symbols represent means (± standard error of the mean). An asterisk indicates that the difference between the height of uninoculated C. atlantica and C. atlantica associated with L. stoechas is significant in the corresponding month according to the Newman Keul’s test (p < 0.05).

Conclusion

13These data show that the management of the mycorrhizal soil infectivity through different cultural approaches (reductionist or holistic approaches) has large potentialities to improve the performances of afforestation programmes, especially in Mediterranean and Tropical areas. This biological tool must be used according to the biological characteristics of the targeted areas (physic-chemical characteristics, biological characteristics) in order to reach sustainable objectives in forest ecosystem productivity and resistance. Hence ecological approaches at community and population scalesmust be encouraged with a view to better informed management of AM fungi in order to propose practical solutions to manage forest ecosystems in a sustainable manner.

Figure 4
Growth responses of
Acacia raddiana seedlings to the soil origins collected under shrub species native from Morrocan arid areas after 4 months’ culture in glasshouse conditions (Unpublished data). HL: Helianthemum lupii; ON: Ononis natrix; HS: Haloxylon scoparium; RR: Retama retama; WA: Withania adpressa; LS: Lavandula sp.: LAS: Launea sp.; CL: Cleome sp.; CT: Convolvulus trabutianus; AA: Artemisia herba alba; AS: Astericus sp.; SHC: Soil non influenced by plants.

Bibliographie

References

Ambriz, E., Baez-Perez, A., Sanchez-Yanez, J.M., Moutoglis, P. & Villegas, J. (2010)
Fraxinus–Glomus–Pisolithus symbiosis: Plant growth and soil aggregation effects. Pedobiologia, 53: 369-373.

André, S., Neyra, M.& Duponnois, R. (2003)
Arbuscular Mycorrhizal Symbiosis Changes the Colonization Pattern of Acacia tortilis spp. Raddiana Rhizosphere by Two Strains of Rhizobia. Molecular Ecology, 45: 137-144.

Augé, R.M. (2001)
Water relations, drought and vesiculararbuscular mycorrhizal symbiosis. Mycorrhiza, 11: 3-42.

Baslam, M., Qaddoury, A. & Goicoechea, N. (2014)
Role of native and exotic mycorrhizal symbiosis to develop morphological, physiological and biochemical responses coping with water drought of date palm, Phoenix dactylifera. Trees, 28: 161-172.

Bossio, D.A., Girvan, M.S., Verchot, L., Bullimore, J., Borelli, T., Albrecht, A., Scow, K.M., Ball, A.S., Pretty, J.N. & Osborn, A.M. (2005)
Soil microbial community response to land use change in an agricultural landscape of Western Kenya. Microbial Ecology, 49: 50-62.

Callaway, R.M. (1995)
Positive interactions among plants. Botanical Review, 61: 306-349.

Callaway, R.M. & Walker, L.R. (1997)
Competition and facilitation: a synthetic approach to interactions in plant communities. Ecology, 78: 1958-1965.

Caravaca, F., Barea, J.M., Figueroa, D.& Roldan, A. (2002)
Assessing the effectiveness of mycorrhizal inoculation and soil compost addition for reafforestation with Olea europaea subsp. sylvestris through changes in soil biological and physical parameters. Applied Soil Ecology, 20: 107-118.

Chaer, G., Fernandes, M., Myrold, D. & Bottomley, P. (2009)
Comparative resistance and resilience of soil microbialcommunities and enzyme activities in adjacent native forest and agricultural soils. Microbial Ecology, 58: 414-424.

Defries, R., Achard, F., Herold, M., Murdivarso, D., Schlamadinger, B. & Desouzair, C. (2007)
“Earth observations for estimating greenhouse gas emissions from deforestation in developing countries”. Environmental Science Policy, 10 (4): 385-394.

Duponnois, R., Founoune, H., Masse, D. & Pontanier, R. (2005A)
Inoculation of Acacia holosericea with ectomycorrhizal fungi in a semiarid site in Senegal: growth response and influences on the mycorrhizal soil infectivity after 2 years plantation. Forest Ecology & Management, 207: 351-362.

Duponnois, R., Colombet, A., Hien, V. & Thioulouse, J. (2005B)
The mycorrhizal fungus Glomus intraradices and rock phosphate amendment influence plant growth and microbial activity in the rhizosphere of Acacia holosericea. Soil Biology & Biochemistry, 37: 1460-1468.

Duponnois, R., Ouahmane, L., Kane, A., Thioulouse, J., Hafidi, M., Boummezzough, A., Prin, Y., Baudoin, E., Galiana, A. & Dreyfus, B. (2011)
Nurse shrubs increase the early growth of Cupressus seedlings by enhancing belowground mutualism and soil microbial activity. Soil Biology & Biochemistry, 43: 2160-2168.

Guissou, T., , A.M., Ouadba, J.M., Guinko, S. & Duponnois, R. (1998)
Responses of Parkia biglobosa (Jacq.) Benth, Tamarindus indica L. and Zizyphus mauritiana Lam. to arbuscular mycorrhizal fungi in a phosphorus-deficient sandy soil. Biology and Fertility of Soils, 26: 194-198.

Manaut, N., Sanguin, H., Ouahmane, L., Bressan, M., Thioulouse, J., Baudoin, E., Galiana, A., Hafidi, M., Prin, Y.& Duponnois, R. (2015)
Potentialities of ecological engineering strategy based on native arbuscular mycorrhizal community for improving afforestation programs with carob trees in degraded environments. Ecological Engineering, 79: 113-119.

Monzon, A. & Azcon, R. (2001)
Growth responses and N and P use efficiency of three Alnus species as affected by arbuscular-mycorrhizal colonisation. Plant Growth Regulation, 35: 97-104.

Ndoye, F. Kane, A., Bakhoum, N., Sanon, A., Fall, D., Diouf, D., Sylla, S.N., Bâ, A.M., SY, M.O. & Noba, K. (2013)
Response of Acacia senegal (L.) Willd. to inoculation with arbuscular mycorrhizal fungi isolates in sterilized and unsterilized soils in Senegal. Agroforestry Systems, 87: 941-952.

Nemec, S. & Vu, J.C. V. (1995)
Effects of soil phosphorus and Glomus intraradices on growth, non-structural carbohydrates, and photosynthetic activity of Citrus aurantium. Plant & Soil, 128: 257-263.

Niering, W.A., Whittaker, R.H. & Lowe, C.H. (1963)
The saguaro: a population in relation to environment. Science, 142: 15-23.

Requena, N., Perez-Solis, E., Azcon-Aguilar, C., Jeffries, P. & Barea J.M. (2001)
Management of indigenous Plant –Microbe Symbioses aids restoration of desertified ecosystems. Applied & Environmental Microbiology, 67: 495-498.

Rodriguez, A. & Sanders, I.R. (2015)
The role of community and population ecology in applying mycorrhizal fungi for improved food security. The ISME Journal, 9: 1053-1061.

Schlesinger, W.H., Raikes, J.A., Hartley, A.E. & Cross, A.F. (1996)
On the spatial pattern of soil nutrients in desert ecosystems. Ecology, 7: 364–374.

Smith, S.E. & Read, D.J. (2008)
Mycorrhizal Symbiosis, third ed. Academic Press, London, UK.

van der Heijden, M.G.A., Klironomos, J.N., Ursic, M., Moutoglis, P., Streitwolf- Engel, R., Boller, T., Wiemken, A. & Sanders, I.R. (1998)
Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396: 72-75.

Weber, J., Ducousso, M., Yee Tham, F., Nourissier-Mountou, S., Galiana, A., Prin, Y.& Lee, S.K. (2005)
Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture. Biology & Fertility of Soils, 41: 233-239.

Table des illustrations

Titre Table 1. Impact of R. irregularis on the growth of tree species in controlled conditions after different times of cultivation
Légende (1) (Shoot biomass of mycorrhizal plants / Shoot biomass of non mycorrhizal plants) x 100. (2) (Root biomass of mycorrhizal plants / Root biomass of non mycorrhizal plants) x 100.
URL http://books.openedition.org/irdeditions/docannexe/image/24051/img-1.jpg
Fichier image/jpeg, 244k
Légende Figure 1Height and collar diameter of carob outplants in the field, either inoculated with AM fungi () or non-inoculated (control ). An asterisk indicates a significant (P < 0.05) difference between the two treatments for a given year (From Manaut et al. 2015).
URL http://books.openedition.org/irdeditions/docannexe/image/24051/img-2.jpg
Fichier image/jpeg, 80k
Légende Figure 2Cumulative mortality of carob outplants in the field, either inoculated with AM fungi () or non-inoculated (control ) during the three years of plantation (From Manaut et al. 2015).
URL http://books.openedition.org/irdeditions/docannexe/image/24051/img-3.jpg
Fichier image/jpeg, 44k
Légende Figure 3Time course changes in plant height (expressed in cm) of C. atlantica outplants growing under natural conditions in the High Atlas Mountains (Morocco), either non-inoculated (Control) () or associated with L. stoechas plants (). Symbols represent means (± standard error of the mean). An asterisk indicates that the difference between the height of uninoculated C. atlantica and C. atlantica associated with L. stoechas is significant in the corresponding month according to the Newman Keul’s test (p < 0.05).
URL http://books.openedition.org/irdeditions/docannexe/image/24051/img-4.jpg
Fichier image/jpeg, 48k
Légende Figure 4Growth responses of Acacia raddiana seedlings to the soil origins collected under shrub species native from Morrocan arid areas after 4 months’ culture in glasshouse conditions (Unpublished data). HL: Helianthemum lupii; ON: Ononis natrix; HS: Haloxylon scoparium; RR: Retama retama; WA: Withania adpressa; LS: Lavandula sp.: LAS: Launea sp.; CL: Cleome sp.; CT: Convolvulus trabutianus; AA: Artemisia herba alba; AS: Astericus sp.; SHC: Soil non influenced by plants.
URL http://books.openedition.org/irdeditions/docannexe/image/24051/img-5.jpg
Fichier image/jpeg, 192k

Auteurs

IRD, France
Microbiologist, IRD, Montpellier, France
robin.duponnois@ird.fr

Cadi Ayyad University of Marrakech, Morocco
Microbiologist – ecologist, Université Cadi Ayyad de Marrakech, Morocco
l.ouahmane@uca.ac.ma

Cadi Ayyad University of Marrakech, Morocco
Soil scientist – ecologist, Université Cadi Ayyad de Marrakech, Morocco
hafidi.ucam@gmail.com

CIRAD, France
Microbiologist, LSTM, CIRAD, Montpellier, France
yves.prin@cirad.fr

© IRD Éditions, 2016

Conditions d’utilisation : http://www.openedition.org/6540